Mr. D. Melegos

Mr. D. Melegos - IDC4U5

Course Description

Welcome to the Grade 12 Biotechnology (University Preparation) website. This course will provide students with the opportunity to explore developments in the field of biotechnology and genomics using laboratory investigations, and inquiry skills. Students will use interdisciplinary approaches to explore theoretical and practical aspects of molecular biology and techniques. Students will study recent developments in genomics, products, research methods, processes, and careers in fields such as medicine, agriculture, and the environment. Students will also evaluate the economic, political, social, cultural, environmental, and ethical issues raised by biotechnology.

Prerequisite – Any University or University/College Preparation Course

Recommendations – SBI3U1

Course Outline [Course Information and Evaluation Policy]

Biotechnology Course Information [Promotional slides about the course content, and evaluation]

Course Flyer [Promotional flyer for prospective students wishing to enroll in the course]

Announcements

Handouts

Introductory Genomics

Genomics Primer [Introductory handout containing information about genomics]

Introductory Genomics [Slide presentation with a focus on introductory genomics and its impact to society]

Molecular Genomics [Handout booklet about DNA replication, DNA transcription, and DNA translation]

Spectrophotometry Lab

Spectrophotometry Lab [Research manuscript guidelines and format about the spectrophotometric analysis lab]

pGLO Lab

Bacterial Transformation with pGLO [Slides that outline bacterial transformation with the pGLO plasmid, and the purification of its product; GFP, using column chromatography and gel electrophoresis]

pGLO Transformation Quick Quide [Resource guide that outlines the procedure of bacterial tranformation with pGLO]

pGLO Transformation Manual [Resource document that contains theoretical and technical aspects of bacterial transformation with pGLO]

pGLO Lab Manuscript Guidelines [Format, manuscript guidelines, and marking scheme for the pGLO lab]

GMO Lab

Diagnosing GMOs [Introductory slides about the processes used to diagnose genetic modified foods]

GMO Quick Guide [Handout refering to the procedure used for the GMO lab]

GMO Manual [Resource document that contains theoretical and technical aspects of the GMO lab]

GMO Pictures [Gel images from past laboratory investigations]

GMO Manuscript Guidelines [Format, manuscript guidelines, and marking scheme for the GMO lab]

PV92 Lab

PV92 Lab Slides [Introductory slides about diagnosing Alu repeats in chromosome 16]

PV92 Quick Quide [Handout refering to the procedure used for the PV92 lab]

PV92 Manual [Resource document that contains theoretical and technical aspects of the PV92 lab]

PV92 Manuscript Guidelines [Format, manuscript guidelines, and marking scheme for the PV92 lab]

PV92 Questions and Answers from past students

Resourses

Column Chromatography [Reverse Phase Column Chromatography of grape soda produced by former DHS Biotechnology students]

DNA Extraction [Video of strawberry DNA extraction]

PAGE of DNA samples [Video of Polyacrylamide gel electrophoresis of DNA samples for the presence of histone proteins]

Bacterial Transformation [Video that contains bacterial transformation with the pGLO plasmid]

Microarray Simulation [DNA microarray simulation from the Dolan DNA Learning Centre]

Microarray Mediabook [An excellent online learning tool about microarray technology and its use]

Extra Help

Extra help is available to students. Extra help can be arranged with Mr. Melegos at a mutually agreeable time. Students are strongly encouraged to take advantage of the extra help available, and to clarify any misconceptions about the course content early in the semester.